Benzodiazepine agonists protect a histidine residue from modification by diethyl pyrocarbonate whereas propyl β-carboline does not
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منابع مشابه
Histidine modification with diethyl pyrocarbonate shows heterogeneity of benzodiazepine receptors.
The effect of diethyl pyrocarbonate modification of histidine on the specific binding of [3H]diazepam and its enhancement with muscimol and (+/-)-pentobarbital was investigated. Diethyl pyrocarbonate treatment produced a dose-related inhibition of specific [3H]diazepam binding to rat brain membranes with a maximal inhibition of approximately 40% at 1 mM. Scatchard analysis of the binding data s...
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C3H mice were immunized with MH134 tumors modified with two different chemicals, DEPC and TNBS, and the tumor-neutralizing activities of the spleen cells were examined after separating the cells by a Nylon-wool column, plastic dish, or treatment with specific antibodies (anti-mouse IgG and anti-mouse theta sera). The effector cells induced in mice by immunization with TNBS-treated tumors were T...
متن کاملInactivation of the endogenous argininosuccinate lyase activity of duck delta-crystallin by modification of an essential histidine residue with diethyl pyrocarbonate.
The argininosuccinate lyase activity of duck delta-crystallin was inactivated by diethyl pyrocarbonate at 0 degrees C and pH 7.5. The inactivation followed pseudo-first-order kinetics after appropriate correction for the decomposition of the reagent during the modification period. The plot of the observed pseudo-first-order rate constant versus diethyl pyrocarbonate concentration in the range o...
متن کاملCircular dichroism studies of diethyl pyrocarbonate-modified histidine in hen egg white lysozyme.
The single histidine residue (His-15) in hen egg white lysozyme (EC 3.2.1.17) was chemically modified by diethyl pyrocarbonate (DEPC) to form exclusively the mono-N-carbethoxyimidazole adduct (second order rate constant of 252 +/- 16 M-1 min-1). Irreversible biscarbethoxylation of the His-15 imidazole ring by DEPC was observed when lysozyme was pretreated with 2-mercaptoethanol (2-ME), 2-ME plu...
متن کاملModification of Histidine Residues of Photosystem II by Diethyl Pyrocarbonate Inhibits the Electron Transfer between the Primary (QA) and Secondary (QB) Quinone Acceptors
The effect o f diethyl pyrocarbonate (DEPC) on the photosynthetic electron transport was investigated in isolated spinach thylakoids by partial electron transport rate and thermolumi nescence measurements. Incubation o f thylakoids at pH 6.5 with 5 mM DEPC for 15 min re sulted in a considerable inhibition o f electron transport from water to dichlorophenolindophenol. The inhibition was only p...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1987
ISSN: 0014-5793
DOI: 10.1016/0014-5793(87)80240-5